A scaled logit model modelling disease occurrence as a function of assay value by an exposure parameter and a parametric protection curve has been proposed, using the logistic function of log-assay value to model the protection curve [ 22].
Antibacterial action of hydroxyapatite discs was studied using a log reduction assay [26].
For high throughput log-phase assays, 48-h cultures in 96-well plates were diluted 30-fold into YPD (∼0.2 of600 of yeast), incubated for 4 h to reach mid-log phase and 100 μl of each well-mixed sample was transferred into 0.2-ml 96-well PCR tubes for heat ramp treatment.
Six animals each received 10 to 1 cell in a log 10 dilution assay.
Following normalization to total protein determined by Bradford assay, log transformation and imputation with minimum observed values was performed for each compound.
For tumour cell motility assays, log phase MDA-MB-231 cells (5 × 104 cells) were suspended in 10 μg/mL control or tamoxifen mammary gland ECM, and overlaid onto transwell 8 μm pore filters as described above.
For spotting assays, log phase cells grown in liquid YPAD were diluted to 1.0 × 106 cells/ml (~0.1 OD units) and 5-fold serial dilutions spotted (5 μl/spot) to YPAD or YPAG plates and incubated for 2 3 days at 18°C, 25°C, 30°C and 37°C.
Quantitative PCR shows a high sensitivity (below 60 cDNA molecules / 10 μl reaction) and dynamic range (8 log units); both within assay and inter-assay variability were below 0.06 log units and accuracy was ±0.06 log units for all chemokines tested in our laboratory using the 5700 Gene Amp Sequence detection system.
17 The limits of detection (LOD) for the RT-qPCR assay expressed in log genome equivalents/mL (log Geq/mL) were 3.8 log Geq/mL (DENV-1), 5.2 log Geq/mL (DENV-2), 3.1 log Geq/mL (DENV-3), and 4.5 log Geq/mL (DENV-4).
For each assay, the log of the fraction growth inhibition was plotted against the log of drug concentration, and the linear regression curve fit correlation coefficient (r value) was calculated.
For each assay, the log of the fractional growth inhibition was plotted against the log of the drug concentration and the linear regression curve fit correlation coefficient (r-value) was calculated.
