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Targeting of the dRecQ4 locus was achieved by a modified procedure described by Rong and Golic [47] and Egli and colleagues [48], [49].
Amplification of this locus was achieved by using seminested PCR.
For example, accurate reconstruction of ancestral genomes at the mammalian CFTR locus was achieved at the DNA level [ 48].
Integration of these expression cassettes into the 230p genomic locus was achieved by double crossover homologous recombination (Fig. S1B C).
Integration of these constructs at the ura3 locus was achieved by selection on plates lacking uracil, and each transformant was confirmed by PCR.
Identification of the genes within the reported deletion locus was achieved using the UCSC genome browser build GRCh36/hg18 at the time.
Similar(53)
Targeted integrations at the maebl locus were achieved in P. falciparum NF54 by single homologous recombination events.
A good frequency of the obtained microsatellite loci was achieved.
Successful genotyping of 239 of these loci was achieved, while the remaining 30 loci presented either difficult interpretation of genotyping data, or absence of amplified products.
When suitable congruence between RG and ML maps for anchor loci was achieved, we applied the resulting order as a fixed order to the mapping of framework markers and all markers.
Homozygosity of this strain for resistant alleles at the Kdr and ace-1 loci was achieved by the F3 generation, as confirmed by the molecular test of Martinez-Torres et al. [ 42] and Weill et al. [ 17], respectively.
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