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All FST values in this study are per locus values.
Permutations of allele copies between populations showed that observed dispersion values are smaller than expected, on average for C and O alleles, as well as for the average locus values, when distinguishing O and C.
To test whether genetic diversity was significantly different between clusters, we applied a Wilcoxon paired-sample test [ 71] on the 10 single locus values of the statistics of interest.
Each individual in the simulation was characterized by 22 unlinked loci with a maximum of 15 alleles per locus (values as those in our dataset) with average mutation rate of 5 × 10-4 and 95% single step mutations [ 41].
To test whether the regression slopes significantly differed between the different parts of a same scale, we used a Wilcoxon paired-sample test [ 71] applied on the 10 single locus values of the regression slope.
Although we can only compare our values with the mean and SD among individual locus values of D xy in the O'Loughlin et al. (2014) dataset, our between-clade values are greater than the mean plus 1 SD of even the gambiae-merus comparison, suggesting that the CTLMA2 values exceed most of the many independent genealogies in their study.
Similar(52)
The relative amount of enrichment was determined as followed: for the input PCR, the experimental locus value was normalized to input act1 + locus value, giving the input ratio.
To obtain confidence limits for the per locus value of FST, 1,000 bootstrap samples were taken each consisting of 1,000 FST values sampled at random from the loci within each data set.
The mutation parameter (θ = 4 Ne μ, where Ne is the effective population size and μ is the neutral mutation rate) was set to a per locus value of 5.0.
Among all populations, the median FST across loci at silent sites is 0.52, with per-locus values ranging from 0 to 0.82 (Fig. 4).
(3) If selection occurs only in one of the two derived populations, extreme deviations for multi-locus values of heterozygosity indicate a selective sweep.
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