Sentence examples for locus targeting from inspiring English sources

Schematic representation of wild-type R26 locus, targeting vector, recombined miR-9-Spfl-Stop allele and activated miR-9-Sp allele resulting from Cre-mediated excision of the 'Stop' cassette.

In summary, our findings lend strong support to the notion that locus targeting by LP-directed protein transduction is both effective and favored by an improved on-target/off-target cleavage ratio.

In S. albus, the PKS-NRPS hybrid gene sshg_05713 from a cryptic polycylic tetramic acid macrolactam gene cluster was chosen to evaluate single locus targeting, again to introduce a short frame-shift deletion using a 2 kb editing template.

An ES clone (RRK001) with the CPD1 locus targeted was used for blastocyst injections to generate chimeras.

The density of markers necessary for analysis will thus depend on the trait, the locus targeted, and the population studied.

The genotyping methods currently used differ with respect to the genetic locus targeted: the SSU rRNA gene, the HSP70 gene, or the GP60 gene.

This reduction may occur not only in the locus targeted by selection but also in the surrounding areas through linkage and genetic hitchhiking [ 9, 10].

To explore the possibility of multi-loci targeting and to see whether additional guides would help reduce the off-target activity, we next transduced our DKOdCas9 cat3a cell line with a pool of ten sgRNAs that target representative loci with distinct methylation and expression levels in wild-type ES cells (Fig. 2a, Supplementary Fig. 3a).

To investigate the underlying causes of multi-locus targeting of microsatellites we performed a BLAST search of these sequences against the Fragaria vesca reference genome (cutoff value 1*E−10).

The other association approach, the least conservative LFMM, identified 75 SNP also being outlier loci, targeting 52 unique predicted genes highlighted in [Additional file 3].

Here we profile the global loci targeted by MLL2.