The assay for the L91 locus was based on oligonucleotides specific for the L91 flanking sequence (andCACACAAGCCCAAAAAGAC-3') and the RB of the T-DNA [ 27].
For all but two SSAP loci, at LG5 and LG6 CO9C-CTC-004 CO9C-CTC-004 CO9C-CTC-004 preference for one pandntal CO9C-AAG-008 CO9C-AAG-008 CO9C-AAG-008 thisfields being distorted in the same direction (Supreferencenformatione Table S4).
When spring Manitou was the recurrent parent, heterozygosity (Vrn1/vrn1) at the Vrn-A1 locus was based on the heterozygotes' flowering time, which was several weeks later than that of the homozygous (Vrn1/Vrn1) spring habit.
When winter Norstar was the recurrent parent, heterozygosity at the Vrn-A1 locus was based on the spring habit, which would be Vrn1/vrn1, due to the dominance of the spring habit allele, whereas all other progeny would be winter habit.
Significance of FST for each locus was based on a test for population differentiation with the log-likelihood G-statistic with the assumption of random mating within samples, implemented in FSTAT.
The probabilities of obtaining states per locus were based on the allele frequencies extrapolated from the control generation, i.e., not from Mendelian except for the control itself (Supporting information: Table S1).
We typed 1423 SNPs that were physically separated by approximately 1 2 kb (Table S1); for these loci, only one locus of the pair was typed on the panel whereas the map position of the other locus was based upon their order in the Btau_2.0 assembly.
Our strategy for the enrichment of methylated genomic loci is based on a modified protocol of Me-RDA and is illustrated in Figure 1A.
The evidence for six loci is based only on single, albeit very large, studies: deletions at 17q12, distal 16p11.2 and 17p12 and duplications at 1q21.1, AS/PWS region and at 16p13.11.
The selection of candidate gene loci was based on three major criteria: (1) Positional candidates: Genes mapping near QTLs for cell wall chemistry [16].
