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Out of the 862 genes deemed to be differentially expressed (363 upregulated and 499 downregulated), we could retrieve the requisite locus information for only 709 genes from the TIGR rice genome database.
For the ≥1.5-fold differentially expressed genes, the locus information corresponding to the Affymetrix probe set IDs was obtained from the National Science Foundation rice oligonucleotide array project (http://www.ricearray.org/matrix.search. shtml) through the rice genome server of The Institute for Genome Research, Rockville, MA (Yuan et al. 2005; Ouyang et al. 2007).
Parsing the input files (CEL and CDF files, mapped reads, locus information) and adding all transformations (as shown in figure 2) took five minutes.
Arrays were normalized using our implementation of the RMA method [22] and locus information for probesets was added from the authors' tabular annotation file.
To investigate whether alterations in gene dosage contributes mechanistically to the differential gene expression identified here in early-stage lung cancer, we compared segmental copy number gain/loss from 20 independent CIS specimens with locus information for up-regulated and down-regulated genes in CIS/SCC relative to BE and PC.
We present SeaSight, a new extension for Mayday, consisting of file parsers for microarray data, mapped sequencing reads and locus information from different sources, as well as a large number of data transformations (platform-specific as well as generic in nature) and operations on locus data.
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With the development of new SSR markers, Brondani et al. ([2006]) could generate a comprehensive consensus linkage map by including SSR loci information from various eucalypts species.
To include multi-locus information in calculating these metrics, we used the mean, for each summary statistics, computed over the 20 autosomal non-coding regions.
When viewing the nucleotides of the two DNA strands as two long rows of code, we consider haplotypes to summarize the horizontal multi-locus information per strand while genotypes summarize the vertical information on a single locus across the two strands.
A multi-locus information index considering the type, number, and position of markers was developed to assess precision.
Additional file 14: Microsatellite loci information.
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