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However, the GUS-expressing locus in line 6b4 is silenced in the presence of the ΔGUS-SUG construct, as evidenced by the loss of GUS activity [30].
To analyze hairpin transgene-induced silencing of a sense transgene, we used the ΔGUS-SUG locus in line 306-1 targetget an expressing GUS locus (Figure 3A).
Based on previous work which demonstrated that deletion of the SACPD-C locus in line A6 elevated seed stearic acid to ~28% [ 12], we anticipated that MM106 bore a deletion(s) distinct from SACPD-C.
One of the two Arabidopsis homologues of YAF9 was shown recently to be required for histone H4 acetylation in the FLC locus, in line with its role as a plant NuA4 subunit [ 16].
Finally, our de novo transcriptome assembly method detected an average of 1.75 transcripts per locus, in line with previous reports using a reference-guided assembly of grapevine transcripts (1.25 transcripts per locus [ 45]).
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These results suggested the existence to two different alleles at the gamma-TMT Tph2 locus in lines nmsT2100 and IAST-1.
The total variation increased significantly with age at all loci in line with the previous analyses (Table S6).
Consider a trait with m ˜ causal bi-allelic loci, where X ˜ i k ∈ { 0, 1, 2 } is the allele content at locus k in line i (the assignment of alleles is arbitrary).
Further evidence for recurrent mutations is the prima facie observation of three alleles at locus R37 in line Vol, where at least one post-origin mutation must have taken place (because the diploid progenitor could not have carried more than two alleles).
In the region spanned by the markers B224C4P2 and Ca_Sb07g023440, the allelic composition of the dwarf inbreds was identical and different from that of the tall inbreds, except at locus Ca_Sb07g023910 in line Tift red (Table 2).
Again, the Igf2-H19 domais is the most studied locus in this line of investigation.
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