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The so identified sequences then function again for later studies as reference and artificially reinforce locus determination.
We were able to score these more divergent loci using synteny data anchoring locus determination and subsequent de novo domain prediction using a combination of 14 HMM algorithms (see Methods).
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Genetic factors underlying vector competence of C. sonorensis for BTV have been explored in the USA and appear to involve a multi-locus determination of susceptibility to fully disseminated infection [ 19].
Single-locus complementary sex determination (sl-CSD) occurs when a single genetic locus determines the sex of the individual that it is found in.
Proof of continuity of the LfHha locus and determination of the intron-exon boundaries was further obtained by PCR amplification on Lf cDNA template of a partial exon1-exon2-partial exon3 sequence (corresponding to amino-acids 38 (R) to 285 (V) of the LfHha protein sequence shown in Fig. 2C).
Sex ratios within crosses were examined for fit to a 1 1 ratio expected under single locus sex determination, using G tests (Sokal and Rohlf, 1995).
This implies that the observed concordance in allelic richness at microsatellite loci and the sex determination locus (∼3 alleles each) in NA populations of L. leucozonium would occur rarely, or not at all, if this species was founded by a larger population which experienced a lag period.
DNA fingerprinting of matched cell lines and patient specimens was performed by Genetic Technologies Corporation Pty Ltd (Melbourne, Australia) using the AmpF/short tandem repeat (STR) Identifiler kit which co-amplifies 15 STR loci and the sex determination locus Amelogenin.
Therefore, we examined all heterozygous loci following determination of genotypes by SNP analysis of total DNA isolated from myoblasts to identify novel regions displaying reciprocally modified chromatin.
If metagenomic data are used to generate a genotype at known genomic positions and with known allele states, the sequencing error has little consequence on a multi-locus genotype determination.
While mapping of the sex phenotype to a chromosome is relatively easy given a single-locus sex determination system, mapping the sex phenotype to a gene has been more difficult, and the master sex determinant has only been identified in a few fish species.
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