Multiple regression analysis was applied to estimate the additive and dominance effects of the FT2 locus, details of which were described previously (W atanabe et al. 2004).
Five loci failed to amplify in the citrus population, six amplified multiple fragments, two were monomorphic, and eight yielded polymorphic loci (for locus details, see Table 2 and Table S1).
Therefore, some isolated cDNA sequences point to a hitherto unidentified allelic variant of an existing HERV-K HML-2) locus, alternatively an, as of yet, unidentified HERV-K HML-2(details to be publisHERV-K HML-2e).
Genotyping was completed for all individuals at 11 microsatellite loci; details of amplification and data collection can be found in Cullingham et al. (2012).
For each lineage-pair, we used all available loci (details on loci number, sequence length, and outgroups available in Additional file 2: Table S1), running each analysis ten times to ensure convergence across runs.
> -wrap-foot> We find the best evidence for the following loci (details in the Supplementary Material): Deletions at 16p12.1 are the most likely finding as it is the only locus significant in the replication dataset on its own and is a known pathogenic locus associated with ID/DD/ASD (14, 16).
References for primer sequences are available in Additional file 1. Reconstructed pedigrees were used to identify animals expected to contribute the most information for linkage mapping purposes (e.g. large sibships and their parents) and these animals were then genotyped at more than 200 microsatellite loci (details below).
We investigated this locus in detail and discovered that its flanking sequence has a GC content of 59%.
Owing to the importance of surface related structures in the pathogenesis of C. jejuni, we decided to investigate the capsule locus in detail in our hyper-invasive strains.
Here we characterize the bmf gene locus in detail, report the molecular basis of the generation of the two major isoforms of Bmf, designated BmfS and BmfCUG, and provide evidence that Bmf can act as a sensor for stress that associates with the repression of the conventional CAP-dependent translation machinery.
