Sentence examples for locus designed from inspiring English sources

DNA sequences were visually inspected to identify perfect and compound microsatellite sequences and PCR primers specific to each locus designed using the program fastpcr [43].

It should be noted, however, that a lung tumor modifier locus (designed Pas3) had been mapped on chromosome 19, in a position slightly proximal to the one herein identified, in an intercross population between A/J and C57BL/6J mice [ 17].

To genotype the cSNPs, an Affymetrix MegAllele Genotyping Human 20 K cSNP was used (Affymetrix), which utilizes a single molecular probe per SNP locus, designed to incorporate two regions of DNA that are complementary to the genomic DNA on either side of the SNP locus.

In the improved genome assembly we identified 158,432 SSR loci, designed flanking primers for 85,296 of these and validated them in-silico by e-PCR.

Multiple microsatellite loci designed from A. palmata [17] are also available for A. cervicornis; however, preliminary analyses of these loci indicate that they are confounded by introgression and homoplasy (Vollmer, pers. obs).

Samples were genotyped at 10 polymorphic microsatellite loci designed specifically for Campylopterus curvipennis ([ 66], GeneBank accession nos. GQ294539-GQ294550).

An additional set of SNVs was identified by intronic primers of further 108 protein-coding loci, designed using Primer3, and purchased from Eurofins MWG (Ebersberg, Germany).

From the original set of eight microsatellite loci designed for A. darlingi from eastern Amazonian Brazil [ 47], one locus (ADC107) did not amplify in any of the individuals collected from Peru and three loci (ADC01, ADC29, and ADC107) did not amplify in any of the individuals from Belize or Guatemala.

The results from the survey of L1 polymorphism using insertion loci from the database are summarized in table 2. Three of the truncated insertion loci designed from the database were also captured by our cloning method, which was not an unexpected result since with that method we were able to retrieve a high proportion of the total copy numbers of the studied L1 families.

We used the primers for these loci designed by Shen et al. (2013) [ 78] and used their nested PCR approach (i.e. an initial PCR for the locus that is followed by a second PCR nested within the region amplified by PCR1).

Most of these studies were conducted within Pinus L. (56 references), which may reflect greater interest in this genus, which is more speciose than other genera in this family, or perhaps may indicate greater success across the genus using loci designed from P. thunbergii Parl.