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Interestingly, this analysis revealed that each genomic locus bound by a given HAT is also bound to some extent by the four other HATs.
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Individual loci bound by single Pol IV or Pol V molecules would likely escape detection in our immunolocalization assays.
We found that most loci bound by Tup1-Ssn6 could not be explained by co-occupancy with a known recruiting cofactor and that deletion of individual known Tup1 recruiters did not significantly alter the Tup1 binding profile.
We then compared the binding pattern to published ChIP-chip data and found that the majority of loci bound by Tup1 were not co-occupied by a known Tup1 recruiter.
This interaction thus possibly explains the rapid acquisition of H3K4 methylation early during iPSC reprogramming at loci bound by ectopic Oct4.
Motif enrichment analysis using the MICRA tool (Southall and Brand 2009) revealed enrichment for the motif ATGCGGG at the loci bound by the Dam-Gcm fusion.
Mean value ± s.e., n = 3. (H ) ChIP-qPCR in N2A cells: POU3F3 occupies a subset of loci bound by Dali and regulated by both Pou3f3 and Dali.
We find a large overlap in their target genes, and indeed with the targets of Prospero, with 245 genomic loci bound by all factors.
With 91 loci bound by the complex, randomly distributed among the blastomeres, permutations of methylation defects could be vast, leading to a wide range of phenotypes.
This hypothesis predicts that fossilized repeat sequences from ancient repeat expansions will be found around loci bound by CTCF in multiple mammals.
The DamID chromatin profiling is a methylation-based tagging method used to identify the direct genomic loci bound by transcription factors (van Steensel and Henikoff 2000; van Steensel et al. 2001).
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CEO of Professional Science Editing for Scientists @ prosciediting.com