For all the outlier data, candidate loci will be annotated by gene ontology database and the functional categories enriched with candidate loci would be considered as potentially selected.
Since rice is a self-fertilizing plant, one must be careful when forming hypotheses about such introgression steps, which would have required at least several back-crossings during domestication and, so-called, the bottle-neck effect, in which rapid genome-wide fixation of unrelated loci would be observed.
In this case, however, putative risk loci would be highly polymorphic because of abundant mutation supply.
Few would currently accept that a system with more than seven loci would be suitable as a universal plant barcode.
As one and only one copy of DNA at all loci would be synthesized during the S-phase, ηr · ΔT = 1, where ηr is the (average) replication efficiency.
If genetic drift or other demographic phenomena correlated with isolation at Easter Island were solely responsible for the significantly different allelic frequencies of MIL2 and MIL3 at this location, we expect that levels of divergence at these loci would be comparable and that other loci would show similar patterns.
If any of the cells types were a single exclusive clone, we expect that analysis using our set of MS loci would be sufficient to detect it, since our group was able to detect clonality of tumor cells in distinct physical locations using a similar set of MS [Frumkin D. et al., submitted].
We used S2FST instead of the FST mean estimator from each group of adjacent SNPs, since a measure of FST mean across an array of loci would be more sensitive to those alleles that reached fixation in the form of the opposite allele, and less sensitive for those fixed in the same direction while variance captures this alternation.
In this regard, further studies using larger samples and investigating additional loci would be of interest.
Previously unknown loci would be expected to be expressed at low levels.
Re-sequencing of these thirteen loci would be needed to distinguish between these hypotheses.
