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A total of 1097 alleles were generated from 307 polymorphic SSR loci, ranging from two to eleven, with an average of 3.6 per locus.
We specifically produced deletions, inversions, and also duplications at six different genomic loci ranging from 1.1 kb to 1.6 Mb with efficiencies up to 42%.
The 19 InDel markers produced the total of 127 alleles for all loci, ranging from 4 alleles (Os06g11600 and 4gAL606639) to 12 alleles (Os07g27590), with the average of 6.68 (Table 3).
The median expected heterozygosity (He) was 0.56, with a wide range of diversity in the different loci (ranging from 0 to 0.84) (Table 2).
The few unique genetic features of B. × rhynchopetala could be due to polymorphism within each parental population (the percentage of polymorphic loci ranging from 37.50 50.32% in B. gymnorrhiza and 16.51 24.62% in B. sexangula).
Table 3 shows that the number of alleles varies between loci, ranging from 3 to 10.
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The polymorphism information content at different loci ranged from 0.217 to 0.825.
The expected heterozygosity (He) at these loci ranged from 0.50 to 0.82.
Three to nine alleles per locus were observed, and the heterozygosity of the loci ranged from 0.381 to 0.905.
The total number of alleles per loci ranged from three to ten; the average number of alleles was 5.43.
Pairwise identity among the loci ranged from 82.0 92.0%, while interspecies identity was 97.7 98.5%.
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