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When this number is equal to or larger then a specificity threshold, t, such significant DNA loci (specific BEs) could be used to construct and analyze of statistically reliable part of the empirical frequency distribution representing relatively high-avidity binding loci of specific TF-DNA binding.
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The script verified loci of age specific variants for the presence of supporting sequence reads (≥5) in both comparison samples.
We used independent, external gene annotations to test the efficiency of our methods for fine mapping of loci to specific causal genes.
The locus of each specific zone shows a continuously or smoothly varying interval in the diffusion-path diagram and corresponds to one of the stable phases existing at the joining temperature.
The total blockade of galantamine-induced neuroprotection in the presence of mAChR inhibitors indicates that these receptors are the primary locus of the specific action of galantamine in the retina.
ZFRs could be used to promote transgene integration at a specific locus, or deletion of specific genomic DNA segments.
estimate of the number of specific loci in the subset of observed 'noise-rich' TF-bound DNA loci.
estimate of the number of specific loci in the subset of observed 'specific- rich' TF-bound DNA loci.
The nucleus image (a) shows live cell CRISPR labelling of specific loci on human chromosomes 1 and 13 and is provided by H. Ma and T. Pederson.
However, both the design and the protein engineering of the ZFs (or TALEs) that are required for the binding of specific loci are time-consuming and costly, and their applications are therefore limited.
Recent advances have carved out new paths leading to novel applications of these genome engineering tools including visualization of specific loci of the genome and targeted regulation of gene expression.
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