Sentence examples for loci of endogenous from inspiring English sources

The annotation includes 21,865 protein-coding genes identified by a comparative approach, 217 loci of endogenous retrovirus-like elements, repetitive elements which comprise about 55.7%% of the whole genome, 99,494 new SNVs, 8,355 new indels, 743,326 evolutionary constrained elements, and 3,182 microRNA homologues.

The gRNAs for each system are designed to target the same locus of an endogenous gene, and comparisons of the birth rate and the mutation rate are conducted.

The targeting vector was knocked into Apln locus for endogenous expression of CreERT2 cDNA (Supplementary information, Figure S1).

Next, we analyzed the effect of gemcitabine on methylation of endogenous loci and first examined global methylation levels.

Epitope tagged strains were constructed as described previously [32], [33] so that open reading frames were tagged at the 3'end of endogenous loci with the GFP-KanR, TAP-KanR, V53-KanR, FLAG3-KanR, or a linker-TAP-KanR cassette.

Our data show that targeted chromatin repression of endogenous loci is attainable but difficult to maintain.

Importantly, heterozygous expression or tagging of endogenous loci did not interfere with cell viability.

These results establish parameters needed to use engineered homing endonucleases for the modification of endogenous loci in plant genomes.

The results reported here constitute a significant step toward the use of engineered homing endonucleases for the modification of endogenous loci in plant genomes.

These results establish a superior approach to Cas9-mediated human genome engineering that enables efficient mutation, repair and tagging of endogenous loci in a rapid and predictable manner.

To date, the number of endogenous loci in Arabidopsis that have been successfully mutagenized with sequence-specific nucleases is low, and it remains to be determined if the observed differences in effectiveness of the two nuclease platforms will prove generalizable.