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There was considerable spread in the loci of collection and notable overlap where both red and green were observed at some gates.
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The frequency of allelism found (16 of 51 mapped loci with existing collection of 350 cloned EMB genes) suggested a level of saturation (30%) consistent with past estimates of 1000 total EMB genes [6].
We used PCR to evaluate the distribution of several of the AES-I loci amongst a collection of 188 P. aeruginosa isolates which was comprised of 35 AES-I isolates (as determined by PFGE), 78 non-AES-I CF isolates including other epidemic CF strains as well as 69 P. aeruginosa isolates from other clinical and environmental sources.
Sequencing of intron presence/absence loci across a collection of D. pulex isolates and outgroup Daphnia species has shown that most polymorphisms result from recent gains, with parallel gains often occurring at the same location in independent allelic lineages [ 96].
Analysis of the composition of the 8 VNTR loci of the S. aureus strain collection revealed that the diversity in the number of repeats varied among the various VNTR loci.
Using this method, HPT length polymorphisms were demonstrated at six out of nine putative phase-variable loci, among a collection of 90 B. pertussis isolates.
As outlined above, targeted resequencing of hundreds of loci in genebank collections is already feasible.
Whereas sequence studies of microsatellites rely on complete or partial genome sequences, typical microsatellite studies of genetic diversity instead use data sets of polymerase chain reaction (PCR) fragment lengths measured for microsatellite loci in a collection of individuals.
Sequence analysis of the bh t locus in a collection of mutant and wild-type tetraploid wheat accessions revealed that a single mutation gave rise to the domestication of 'Miracle-Wheat.' As a result of branch formation, this mutant allele produces significantly more grains per spike, leading to higher spike yield.
The studied the polymorphism in 5 VNTR loci using a collection of 150 isolates and found 76 different MTs.
A recent microarray-based approach aims at identifying transcribed HERV loci by a collection of locus-specific oligoncleotides (Perot et al. 2012).
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