Southern blot was performed to determine the number of transgene copies inserted in each single-locus line.
Previous researches found that the ALVE susceptibility between line 63 and line 72 should be dependent on allelic differences at the receptor TVB locus (line 63 is susceptibility SNP TVB-S and line 72 is resistant SNP TVB-R) [33], [47].
Although their work did not specifically address a DNA barcoding approach, Feliner and Rosseló [101] persuasively argue in favor of a multi-locus line of attack if ITS2 is to be used for assessing organismal diversity.
Data reported in the previous section suggests that about 25% of the silenced lines will be silenced mainly at the 5XUAS LUC); a potential advantage to using the single locus line as recipient is the potential for less silencing in subsequent generations for the 5XUAS rsGFP-GUS (e.g. [ 25, 26].
In such cases, a consensus genotype was established for the corresponding loci-line combination based on the genotypes of the three individuals scored.
Segregation analysis of transgenic offspring in the T1 generation revealed that five lines harbour one T-DNA locus (lines 3, 6, 8, 13 and 14).
Plant line K-13 showed the lowest expression (10-fold reduction), while the two single insert locus lines, K-4 and K-16, showed 5-fold and 0.75-fold lower relative expression levels relative to that of the four insert loci K-10 line.
It may be interesting to compare the number of unmethylated LINE-1 loci with LINE-1 methylation levels in other clinical samples.
The total variation increased significantly with age at all loci in line with the previous analyses (Table S6).
On a practical level, because of the homozygosity of most loci, F2 lines resemble recombinant inbred lines and should be genetically uniform, facilitating mapping and cloning of genes responsible for the associated traits.
To analyze hairpin transgene-induced silencing of a sense transgene, we used the ΔGUS-SUG locus in line 306-1 targetget an expressing GUS locus (Figure 3A).
