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With the development of new SSR markers, Brondani et al. ([2006]) could generate a comprehensive consensus linkage map by including SSR loci information from various eucalypts species.
Additional file 14: Microsatellite loci information.
Disease loci information was gathered from the OMIM online database (downloaded on Mar 25, 2009) [ 31].
The results contain genomic loci information of gRNAs and SNP/INDEL inside them.
Loci information was described in Additional file 1: Table S1 in the Additional file of Supporting Information.
Finally, gene and loci information can be reached with links to other browsers such as Ensembl, UCSC and NCBI.
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Out of the 862 genes deemed to be differentially expressed (363 upregulated and 499 downregulated), we could retrieve the requisite locus information for only 709 genes from the TIGR rice genome database.
For the ≥1.5-fold differentially expressed genes, the locus information corresponding to the Affymetrix probe set IDs was obtained from the National Science Foundation rice oligonucleotide array project (http://www.ricearray.org/matrix.search. shtml) through the rice genome server of The Institute for Genome Research, Rockville, MA (Yuan et al. 2005; Ouyang et al. 2007).
To include multi-locus information in calculating these metrics, we used the mean, for each summary statistics, computed over the 20 autosomal non-coding regions.
Parsing the input files (CEL and CDF files, mapped reads, locus information) and adding all transformations (as shown in figure 2) took five minutes.
Arrays were normalized using our implementation of the RMA method [22] and locus information for probesets was added from the authors' tabular annotation file.
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