Sentence examples for loci expression vector from inspiring English sources

The recombinant human, rat, or dog miR-433/127 loci expression vector (designated pMIR-REPORT-NoMp-human, rat or dog) was then transfected into Hepa-1 cells and the expression of miR-433 and miR-127 primary transcripts was examined using semi-quantitative RT-PCR.

In this work, we explore the suitability of a BAC containing the Rosa26 locus as expression vector applied to the production of the Fc fragment of the constant region of human IgG1 in HEK 293 cells.

In vitro over-expression of the human, rat, or dog miR-433/127 loci in cells, using an expression vector containing miR-433/127 and their promoter regions, markedly induced a differential expression of both primary and mature miR-433 and miR-127, indicating that miR-433 and miR-127 were possessed from their independent promoters.

These effects can be avoided by stable integration of single copies of each expression vector into established loci in the mouse genome.

CkbTg mice were generated by over-expression of Ckb under control of the human CD2 promoter and locus control region in the transgenic expression vector p29△2 (gift of Paul Love), two transgenic founders with similar phenotype were generated and one line was used in this study.

However, two lines that expressed the full-length protein also failed to produce gametophores, possibly due to mistargeting of the expression vector to a separate locus required for gametophore development.

HmLZ-IRES2-EGFP fragment with NheI enzyme loci at 5 'end and EcoRI enzyme loci at 3′ end was amplified from the recombinant eukaryotic expression vector pHmLZ-IRES2-EGFP using primers LYZ-F1 and LYZ-R1 (Table 1).

Next, Tg mice were created using a 'half-genomic' construct expression vector derived from the mouse Prnp locus (Borchelt et al, 1996; Fischer et al, 1996).

The Chlamydomonas BafR1 mutant strain with a complete gene deletion at the STA2 locus that encodes GBSS [18] was transformed with the GBSS-PfMSP1-19 expression vector.

The newly constructed expression vector pSAK was designed for integration into the α β tubulin locus, which is tandemly arranged in the T. congolense genome.

TALE arrays listed in Supplementary Methods were cloned into a TALEN expression vector bearing TALE domain truncation points that enable genome editing activity at endogenous loci.