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SAF-B interacts with DNA dependent upon its SAP DNA-binding domain, with other chromosomal loci dependent on RNA, and is recruited de novo upon induction of transcription.
Some studies have investigated age-associated methylation of CpG loci dependent on sex, body mass index, specific tissue, or cell type [ 8– 12].
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The cryptic promoter in the LTR of the IAP causes aberrant expression of the Agouti locus dependent on its level of CpG methylation.
One possible explanation is, that transgene silencing effects [ 28] also occur in ZFHD1::miceT1 mice and that the accessibility of the target promoter is also gene locus dependent.
The high values of FIS obtained were not locus dependent, indicating the markers used did not display technical issues such as null alleles.
Recombineering represents a powerful and scalable method for determining gene function and for exploring how locus dependent and independent parameters contribute to the efficiency of in vivo homologous recombination in Drosophila.
Thermocycling conditions included an initial denaturation step of 5 min at 96°C followed by 30 cycles each with 96°C for 30 s, Annealing at 52°C-58°C 52°C-58°Cpendent, Tablocus) for 30 s, andependentr 30 s.
As is the case for many ascomycete fungi such as Neurospora crassa (Ninomiya et al., 2004), Stagonospora nodorum (Feng et al., 2012) and Magnaporthe oryzae (Kershaw and Talbot, 2009) the gene targeting efficiency in Z. tritici is often low and locus dependent (Bowler et al., 2010) and is thus not amenable to a medium or high-throughput functional genomics analysis.
In addition, targeted integration in strains deficient of NHEJ proteins can be highly locus-dependent and may vary from 33 to 100%% depending on the insertion site (Jørgensen et al. 2014; Schuster et al. 2012).
Some studies conflict or suggest that methylation-age associations are tissue- and locus-dependent (Eckhardt et al. 2006; Heijmans et al. 2007).
Collectively, our data suggest that rRNA gene activation can occur in a gene-autonomous fashion, independent of chromosomal location, whereas rRNA gene silencing in nucleolar dominance is locus-dependent.
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