Sentence examples for loci check from inspiring English sources

Antibiotic resistance was removed by the expression of FLP recombinase from the pCP20 plasmid, and successful deletions were confirmed by loss of antibiotic resistance and by PCR using loci check primers (Table 2).

2) Clustering of RNA loci, checks for other RNA loci annotated in the vicinity of the one in question.

More specifically, the three copies of icmF are transcribed from their respective loci, impC is expressed from both loci checked, and impJ/vasE is expressed from its single locus, while RT-PCR did not reveal detectable transcription for the vasG/clpV genes at both loci examined (Fig. 4).

Following sequence selection and preliminary analyses, the protein sequences for both sets of orthologs were compared to their genomic loci to check ORF and intron calling.

We performed bootstrap analyses of each individual loci to check for compatibility of their individual phylogenetic signal.

As a further scan for paraloguous sequences, we re-blasted the full genome of Callorhinchus milii against the remaining 174 loci to check, if each sequence has only a single hit in the C. milii genome using customized Perl scripts (Additional file 1).

Out of 15 loci randomly checked for polymorphism, 13 loci were polymorphic with allele number ranged from 3 to 6 and two loci were found to be monomorphic.

Classical 3C can detect chromosome interaction between two interested loci by checking the ligation products with PCR using locus-specific primers (one to one).

The syntenic loci were checked to see whether SINE1 was present there in both species.

The absence of recombination in the 13 nuclear loci was checked using the Φ test [ 48].

The loci were checked for large allele dropout, stuttering, and null alleles using 1000 randomisations in MICROCHECKER version 2.2.3 [ 79].