Sentence examples for loci and methods from inspiring English sources

From these empirical results we determine if the numbers of loci and methods used influence phylogenetic inference for pseudoxyrhophiines.

To characterize the rare genotypes capable of suppressing gal7 Δ-dependent galactose toxicity, we used RAD-seq to individually genotype 247 galactose-tolerant progeny of the EC33-gal × YPS163-gal cross at 469 loci (Materials and Methods).

Comparisons are made between S2 cell technical replicates 150a and 150b (A ) Variability of log2 fold-changes in ribosome footprint densities are no greater for multi-isoform loci (pink) than they are for single-isoform loci (blue) (B ) Correlation of the fraction degenerate positions in each locus ('Materials and methods') with fold-changes in ribosome density between replicates at that locus.

To assess if any of these ORFs are translated, gene fusions with the GFP-encoding gene were generated and integrated into the P. marneffei genome either at the ystA locus or at the pyrG ectopic locus (Materials and Methods section).

In this study, we address the question of pleuronectiform monophyly with a broad set of markers (from six phylogenetically informative nuclear loci) and inference methods designed to limit the influence of phylogenetic artifacts.

To disrupt the mouse coronin-1A gene, we employed gene-targeted mutational techniques to delete genomic DNA sequences from the corresponding locus (Materials and Methods S1 and Figure S1A).

(A ) Schematic of constructs and crosses utilized for accelerated homologous recombination of the Or7a locus (See Materials and methods for details).

We generated random mutations by using PCR and replaced the wild-type copy of RPB2 with the mutant alleles via plasmid shuffle in a yeast strain deleted for the chromosomal RPB2 locus (Materials and Methods).

To fate-map Cux2-expressing cells in the hippocampus, we utilized a Cux2-ires-Cre line that has an IRES-nuclear localized Cre cassette inserted in the 3′UTR of the Cux2 locus (Materials and Methods).

> -wrap-foot> aTwo candidate fathers were both excluded at a single locus, while all other males were excluded at multiple loci (see " Materials and methods").

This will involve the powerful combination of alignment-free methods to predict putative homologs in the orthologous gene locus and experimental methods to characterize the expression readout of the predicted homolog.