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The first latency times likely reflected the method of drug administration employed in this study (drugs were added to ACSF), the speed of ACSF perfusion (1 ml/min), the volume of the recording chamber (∼2 ml), the location of recorded neurons within the slice and the location of slices within the recording chamber.
Across the CSD profile, the deepest current sink corresponds to the thalamo-recipient granular layer (Kaur et al., 2005; Szymanski et al., 2009) allowing us to reconstruct the laminar location of recorded neurons.
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Fresh-frozen brains were cryostat-cut into 20 µm coronal sections for further validation of the location of recording track into the GP, STN and SNr as previously described [15], [18].
(C ) Location of recording sites as in (B ).
We pooled cells in this way because it was difficult to define the precise location of recording electrodes on each particular recording session.
(A ) Brain atlas image (Paxinos and Franklin, 2013 ) illustrating bilateral tetrode implantation (top) and location of recording electrodes in Ctrl and KO mice.
Reversal of slow-wave polarity in the LFP depth profile comes then as natural consequence of differential location of recording electrode relative to the dipoles of large pyramids from deep layers.
White inverted 'T' shape denotes location of optetrode track (vertical line) and approximate width of optetrode (horizontal line), which estimates the medial-lateral range of potential locations of recorded single-units.
(B ) Locations of recording sites across the auditory cortices of four female and four male subject rats.
Anatomical data is unavailable for the precise locations of recording sites because animals are still being used in related experiments.
DOI: http://dx.doi.org/10.7554/eLife.03185.008 10.7554/eLiFigure85.009 Figure 3 figure supplement 1. Locations of recording sites in the auditory cortex and cell-type classification.
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