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We subsequently examined whether the location of mutant residues in the three-dimensional CBS structure in respect to solvent accessibility is a determinant of their properties.
There was no clear correlation between the location of mutant proteins in different domains and their misfolding with the exception of mutant proteins in the carboxyterminal domain that were mostly assembled correctly.
However, we can appreciate the reviewer's confusion with this figure, and have added a sentence in the Materials and methods to clarify that these data indicate the relative tissue location of mutant and wild-type bacteria at the time of our assays.
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c Subcellular location of mutants of X12345 in BL21 DE3) after lactose induction for different time periods.
Mutation-based fault localization (MBFL) is a recently proposed fault localization approach via mutation analysis, which uses the location of mutants to identify the faulty statements.
To extend these observations, we determined the location of mutants BmH38RwbkF, BmH38RwbkD (both of pattern 1), Bm16MRwa** (pattern 3), and BmH38RmanBcore (pattern 4) 0.5, 2, 4, 8, 12, and 24 hours after infection.
The location pattern of mutant VER2-S33G is different from that of VER2.
Although 4-PBA facilitates the degradation of T17M mutant and attenuates apoptosis induced by ER stress, it does not affect the location of T17M mutant.
The mechanistic basis for additive/mitigating effects cannot be solely due to total mutant gene dosage alone, but also appears to be highly dependent on the population and location of double mutant regulatory units along the thin filament and the biophysical properties of the effected sarcomeric protein in terms of the activating/deactivating nature and the strength of these mutant alleles.
Bulked segregant analysis (BSA) by pooling equal amounts of DNA from 20 short-root or 20 normal-root plants was used to determine the chromosome location of the mutant gene.
An additional advantage of the LI-COR system, is that the use of different dyes for each primer allows a precise estimation for the location of the mutant.
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CEO of Professional Science Editing for Scientists @ prosciediting.com