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On the one hand, using fluorescent chemical probes targeting various subcellular compartments, we and others reported that dyes located at the PM rather than intracellular ones were transferred, suggesting some selectivity based on the location of labels within the cell [7], [8].
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At the location of label C the segments merge again.
These images reveal tantalizing details of the cytokinesis process and are the first steps in our goal of generating a portfolio of tomographic images that map the location of labeled molecules into high-resolution 3-D reconstructions of the cell.
Bright-field images of the fluorescent axons in the SN were used to estimate the location of labeled axons (A, bottom layer).
To reveal the precise location of labeled neurons and neuronal projections to the cortex, we counterstained them with 0.5% Neutral red (Sigma, St . Louis MO, USA).
We examined the location of labeled neurons in area X (resulting from the injection in area Y) with respect to the injection site in area X (from a different case) that failed to label neurons in area Y.
Because the uptake zone of these tracers is defined and restricted, it is possible to determine the number and spatial location of labeled intrinsic neurons (see technical section of Discussion and Supplementary information).
Thus, before the patch-clamp experiments, the morphology and location of labeled fibers was carefully examined in order to exclude the possibility of activating parasagittal long-range Lugaro axons or nearby Golgi axons.
The locations of label edge routers depend on the demands of a given set of terminal networks which in turn affect the design of the backbone network.
The ultimate goal is to identify the locations of label-edge routers and label-switching routers, and to determine the interconnection links and their capacities to accommodate expected traffic demands.
Where comparisons between patches and cortical function have been made, a consistent albeit weak correlation between the locations of labeled patches and markers for cortical function has emerged.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com