The idea for the space-saving stacked interchange is attributed to WH Irish, a location engineer with the California Division of Highways in the 1940s.
To study the effects of binding site location, we engineered TALEs customized to recognize specific DNA sequences located in either the promoter or the transcribed region of reporter genes.
To explore the clinical significance of repeat location, we engineered a variant of the model protein with a polyQ tract within the domain, a location that does not mimic physiological context, demonstrating significant destabilization and structural perturbation.
To make streptokinase fibrin specific and limit the fibrinolytic process to the clot location, we engineered a chimeric streptokinase by fusing the fibrin binding Kringle 2 domain of tissue plasminogen activator (TPA) to the streptokinase N-terminal end.
To confine cell adhesion to specific locations, an engineered surface for high-contrast protein adsorption and, hence, cell attachment has been developed.
"By checking the engineers' location against time, we have managed to reduce engineers' overtime claims significantly since engineers are notorious for adding a few minutes or hours to a day and claiming it as overtime".
Knowledge of that final resting location would enable engineers better to understand its predicament and the prospects for future contact if lighting conditions somehow change on 67P.
The remote location also prompted engineers to assemble the Correlator in four stages, with the first part of the machine arriving in Chile in 2008.
In fact, threats to expatriates and locals working in difficult and dangerous locations – whether commercial engineers or aid workers, doctors or security contractors – are now more common than in any period I can remember in the past 30 years.
About 575 feet of the line is totally collapsed, in two separate locations, but subway engineers have said that hundreds more feet are structurally unsound, with once-straight I-beams bent into curves and dozens of holes punched through the tunnel walls by falling debris.
