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Likewise, our expression analysis and subcellular location assay indicated that OsLAP6/OsPKS1 has a tapetum-specific expression pattern and is localized to the ER (Figs. 5 and 6), and cytological observation revealed the defective exine formation in oslap6 mutants (Figs. 2 and 3).
In order to determine if Wolbachia infection affects WCR larval competitiveness, a host location assay was conducted.
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Location assays have indeed identified that a significant proportion of nuclear receptor binding sites are distant from the regulated genes [43].
The genome-wide location assays reported by Harbison et al. [ 16] were used to identify transcription factor binding.
To show the functionality of OLEO as an anchoring protein to immobilize TCII in cell membrane structures after their transgenic expression, we made co-location assays of the expression of the triple fusion protein GFP-TCII-OLEO by immunostaining of either calreticulin, a protein of ER membrane, or golgin 97, a protein of Golgi apparatus, as described previously in COS-7 cells [16].
BES compared to WGS traces showed polyploid-like regions with less than 1% sequence divergence exist at 2.3% of the locations assayed.
Characteristics that may bias the assessment of RE DNA methylation include evolutionary age,, genomic location, and assay method.
In all assays, samples were randomly distributed across plates to avoid confounding due to the plate location, and assay operators were blind to the disease status or time point of samples.
Expression of heme oxygenase-1(HO-1) and Nuclear factor-erythroid 2-related factor 2 (Nrf2) and Nrf2 cytoplasm nucleus location were assayed.
PAM was equally active when targeted to each intracellular location and assayed in homogenates.
Vulva location was assayed as follows: the number of times a male passed the vulva before stopping was noted.
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