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A-well known catalytic residue Cys215 is located on the loop that stays at the edge of this β-sheet.
222C to G or T substitution was located on the loop structure at domain IIIa of HAV IRES.
We found that the residues in the potentially unreliable regions were mainly located on the loop regions in the computational model, indicating that further molecular dynamics optimizations are needed, as described below.
Glu is located on the loop between β2 and α2.
This residue is located on the loop between α3 and β4.
D299G is near the convex part, while T399I is located on the loop C-terminal to the convex part.
Similar(51)
The best thermostability improvement (16%) was observed for Mut4 in which two hydrophilic residues located on the loops, far from the active site, were replaced by Valines.
Previous reports on the anti-PD-1 MAbs revealed that the binding of these MAb is mainly located on the loops of PD-1, i.e., the N-terminal loop of PD-1 for nivolumab interaction and the C'D loop for pembrolizumab.
The identified active peptides are located on the loop-β-sheet region of the protein.
Inspection of the LF's crystal structure revealed that residues K329C and S307C are located on the loops on either side of α-helix M and that Y274W is located on α-helix L, near S307C.
Possible subsite residues Tyr30 and Tyr32 and catalytic residue Glu82 along with Met57 of CgPAD are located on the β1-β2 loop and β3-β4 loop, respectively (see Additional file 4 A).
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