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The anti-hDAT-EL2 antibody (Chemicon, AB5802) recognizes an epitope of 18 amino acids (NH2-CHLHQSHGIDDLGPPRWQ) located in the 2nd extracellular loop, while the anti-hDAT-Ct antibody (Chemicon, AB1766) recognizes an epitope of 22 amino acids (NH2- CEKDRELVDRGEVRQFTLRHWL) in the intracellular C-terminal region.
It has been known that the homeotic expression of MnSOD is regulated by AP-2 and SP-1 binding to the promoter of MnSOD gene [ 29], whereas induced expression of MnSOD requires enhancer activation located in the 2nd intron [ 30].
Glu98 4, which is also located in the 2nd shell of the metal center and hydrogen bonds with Nɛ2 of His104, on the contrary, is not required for metal cofactor binding.
Using the degenerate primers SU80a [ 69], SU149a, SU161a, SU193a [ 42] or SU200Ca, combined with SU304b [ 15] or SU306b [ 42] we amplified a gene fragment coding for residues from aa sites 81 94, located in the 2nd α-helical transmembrane region of the SWS1 opsin.
With the same protocol and primers as are described in [ 3] and [ 26] we then amplified a fragment of the SWS1 opsin gene, containing the aa residues at positions 81-94, alocatedted in the 2nd α-helical transmembrane region.
Printers for long-term visitors are located in the 2nd floor hallway and on the 3rd floor in room 317.
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In 2004 the museum opened a second site, located in the 17th-century Baroque Köpenick Palace.
This suggests that regulatory elements could be located in the 3′-UTR.
All top level functions of S4 are located in the S4 library.
Auditions on 09/01 will be located in the 220 York Chapter Room, and auditions on 09/02 will be located in the 220 York Ballroom.
After closing for a restoration project last June, the Beaux-Arts-style Heckscher Museum of Arthelocated in the 18.5-acre Heckscher Park, reopens in March.
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