It is expected that this uORF would drastically inhibit translation from the first SPG4 AUG, while allowing re-initiation at the second AUG that has a G in position +4 and is located at a sufficient distance from the stop codon of the uORF.
The haptic environment had a simulated mass of 5.0 kg and was critically damped in all directions; virtual stops were located at a distance of ±100 mm from the neutral position to ensure the safety of subjects.
A fragment encompassing human tail 1 excluding the 3' UTR was PCR-amplified from pEYFP-hDMPK A with forward primer 5'-GGAAGATCTGCTGTCACGGGGGTCCC-3' adding a BglII site (underlined) and reverse primer 5'-CGAATTCTCAGGGAGCGCGGGCGG-3' located at the stop codon containing an EcoRI site (underlined).
Thanks to this information, the GSCAs located at each stop do not have to carry out time-consuming searches for the GSPAs available at each stop.
The log likelihoods show that uORFs show a marked propensity to disfavour the strong, common signals located at real ORF stop codons, and they show a preference for 12 out of the 15 "leaky" weak termination signals.
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In addition, since 1984 it has erected 20 McStops - McDonald 'slocated at truck stops.
Ventra cards cost $5 at vending machines which are located at all "L" stops.
The design would allow passengers to also be taken directly to their destination of choice, instead of relying on fixed stops, and summoning vehicles would be handled either by phone (likely via app) or fixed summon buttons located at traditional bus stops.
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