Exact(3)
Indeed, more trans-acting factors are known for osk than for any other localized transcript [16].
Using direct RNA FISH, it is also clear that the level of expression of DXZ4 differs from cell to cell within the same cell line; with some cells showing little to no expression whereas others may show DXZ4 localized transcript patterns comparable in size to that of XIST RNA.
Some of the observations that we made based on the genome wide data may seemingly only confirm prior assumptions about the nature of RNA regulation (such as the length and conservation of UTRs in localized transcript).
Similar(57)
To address whether oogenesis localized transcripts remain localized into early embryogenesis, we extended the localization dendrogram to stage 1 3 (maternally loaded transcripts) and stage 4 5 (after the onset of zygotic transcription) of embryogenesis.
Based on these data, we estimate that around 1% of the Drosophila genes encode for germ plasm-enriched, localized transcripts.
We conclude that this combination of microarray and in situ hybridization techniques is a simple but powerful experimental design for the genome-wide identification of genes coding for germ plasm localized transcripts.
Hybridizations were performed with antisense probes to localize transcripts and sense probes as a negative control.
To localize transcripts, we performed RNA in situ hybridization on stems.
In situ hybridization localized the transcript to the fetal renal glomerulus.
Bases that were somatically mutated in cancer and recorded by COSMIC were localized on transcript sequences and their proximity or inclusion to the 17 base NlaIII tag was determined.
We performed an in-depth analysis on orf21 transcript and on its product, RPHM21 (from R. philippinarum Male mitochondrial protein 21): we localized orf21 transcripts by in situ hybridization (ISH), and synthesized a specific antibody to verify the existence, by Western blotting and confocal microscopy, of RPHM21 in adults and early embryos.
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