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First, the model is applied to a synthetic alluvial-fan environment downstream from a localized contaminant source in order to illustrate the model behavior.
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Replacing the filter-based detectors with a hyperspectral imaging system has been shown to identify and eliminate sources of error, such as spatially localized contaminants [ 1]. Background fluorescence can be removed by post-processing, or reduced via structured illumination of the sample [ 2].
The fluorescence contribution of the contaminant cannot be corrected with current commercial technology due to extreme spectral overlap and the variability of the spot-localized contaminant concentration.
For example, a maximum of 100 arbitrary fluorescence units of spot-localized contaminant signal would require a threshold of 500 arbitrary fluorescence units be set in an attempt to maintain errors in the 20% range.
Unfortunately the effect of spot-localized contaminant emission cannot be corrected in commercial scanner microarray data using standard background correction procedures that estimate background signal for a spot from the signal around the spot and thus it leads to errors in the calculated expression ratios (R/G).
This is indicated that the migration and distribution of the contaminants species are still localized and not diffused with a wide area.
While higher concentrations of contaminants can be found by sampling localized hot spots, the NST data represent the distribution of concentrations over general areas of the coastal United States.
The main goal of its application was to confirm the results obtained using SRFTIR microspectroscopy and to verify whether the observed creatine deposits were localized within the analyzed tissue and were not laboratory contaminants.
Similar approaches may be useful in identifying CECs relevant to hazardous waste sites, provided they differentiate between contaminants that are widely dispersed at low concentrations and those that are localized at field sites (Table 1).
Moreover, because of the occurrence of dual localizations of proteins, e.g., of Fis1 and Rho1, which are dually localized to mitochondria and peroxisomes, or Pex30, which is present in peroxisomes and the ER, certain proteins can erroneously be regarded as contaminants.
Others are localized.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com