The interaction mechanism of EWS involves the N-terminal and centrally localized amino acids, while for optimal association full-length EWS molecules are required [ 24].
Usually two nuclear localization sequences (NLS) localized between amino acid residues 320 and 405 were supposed to represent the inter-domain region between MTase and RdRp (Brooks et al., 2002).
Supported by mathematical calculations, we designed a high-density mutagenesis procedure for the generation of a mutant library of which a limited number of random clones would suffice to exactly localize amino acid residues essential for a particular protein-protein interaction.
Double immunostaining was performed for β-galactosidase and markers of kidney tubules to localize retinoic acid activity.
The first splice variant of nrnp1 encodes a polypeptide with 344 amino acid residues; the RRMs are localized at amino acid residues 76 to 132 and 150 to 219 (Additional file 1: Table S2, Additional file 1: Figure S6A).
The LUMO of D1 locates on benzothiadiazole rather than cyanoacrylic acid anchoring groups, while the LUMOs of D2 and D3 are localized on cyanoacrylic acid.
MHC alloantigens are therefore similar to multideterminant protein antigens that bear localized immunodominant amino acid sequences that trigger immune responses toward the whole protein molecule [22] [25].
As a candidate for the glutamine fluxes, the root tip localized, putative amino acid transporter CAT8 was analyzed and heterologously expressed in yeast and oocytes.
The KGDH with its lipoic acid localized in the E2 subunit would be a natural sensor of intracellular oxidative tension [19].
