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The fluorescence images and the calculated co-localization values suggest that both isoforms not only localize to the nucleus but also show similar colocalization pattern with important nuclear proteins.
These relative K5K8 co-localization values were means calculated based on the analysis of a minimum of 6 sections of each thymus derived from 5 independent experiments (N = 30).
Co-localization values were far lower for Par14-EGFP (11 ± 6%) and EGFP alone (4 ± 1%).
The transfected constructs and co-localization values are schematically depicted in Figure 3D.
Processing of the pictures and calculation of co-localization values were all performed within the Cell^P software.
EGFP fusions of the two Par17 isoforms, Par17-QR and -RS, were targeted to mitochondria with no significant differences in their co-localization values with MitoTracker.
In this process, F-actin/G-actin transition was particularly dynamic, observing co-localization values between ENO and F-actin up and down.
The percent co-localization value of eGFP NPRA with Rab 11 showed that receptor recycling occurred at 5 min (13%), 10 min (19%), 15 min (17%) and 30 min (5%; Figure 4B).
Significantly enriching GO annotations were identified for four key compartments or processes; peroxisomal organization regulators (p value: 4.68E-22), mitorganizationregulatorsn regulators (p value: 2.90E-06), cytoplocalizationization (p value: 9.38E-07) and serine/threonine kinases (p value: 6.52E-06).
This distortion is noticeable even over relatively short traveled distances, and is mainly caused by the dispersion phenomenon among other reasons, therefore using conventional localization/multilateration methods will produce localization error values that are highly variable and occasionally large.
Co localization R values (Pearson's correlation) were quantified with co localization module of Volocity5.2.1.
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