The percent co-localization value of eGFP NPRA with Rab 11 showed that receptor recycling occurred at 5 min (13%), 10 min (19%), 15 min (17%) and 30 min (5%; Figure 4B).
The fluorescence images and the calculated co-localization values suggest that both isoforms not only localize to the nucleus but also show similar colocalization pattern with important nuclear proteins.
These relative K5K8 co-localization values were means calculated based on the analysis of a minimum of 6 sections of each thymus derived from 5 independent experiments (N = 30).
The transfected constructs and co-localization values are schematically depicted in Figure 3D.
Co-localization values were far lower for Par14-EGFP (11 ± 6%) and EGFP alone (4 ± 1%).
Processing of the pictures and calculation of co-localization values were all performed within the Cell^P software.
EGFP fusions of the two Par17 isoforms, Par17-QR and -RS, were targeted to mitochondria with no significant differences in their co-localization values with MitoTracker.
In this process, F-actin/G-actin transition was particularly dynamic, observing co-localization values between ENO and F-actin up and down.
The AF647-phalloidin probe yielded 930 photons per activation event (Supplementary Figure S6e), resulting in an inferior localization precision value (17 nm, Supplementary Figure S6f).
Moreover, the number of colocalizing 2HOM blocks in each species (excluding P. cinnamomi) is significantly higher than expected by random localization (P value < 0.0001; supplementary Materials and Methods, Supplementary Material online).
Module 1 is the largest with 473 genes of which 21 are involved in cell transport (p-value 2.75e-07 2.75e-07 involved in nucleotide binding (p-value 1.53e-04) and 19 are involved in protein localization (p-value 3.60e-04).
