Sentence examples for localization validated from inspiring English sources

Four proteins were found in the acidocalcisome proteome and their localization validated by IFA using epitope-tagged proteins: vacuolar H+-ATPase subunits a and d (TbVA a and TbVA d), acid phosphatase (TbAP), and phosphate transporter (TbPho91).

Enrichment of proteins residing in the same or related subcellular localization validates the biological coherence of our predictions.

Bioinformatic analysis of the resulting peptides allowed the identification of a set of candidate proteins whose acidocalcisome localization was validated by in situ epitope-tagging and western blot analyses using specific antibodies (Fig. 1 ).

The large number of putative lipoproteins identified in this genus and combined knowledge available regarding their localization (experimentally validated as well as predicted based on function or domain content), however, provided a more sensitive means to search for conserved sequences that are characteristic of surface lipoproteins.

By performing co-immunoprecipitations and studying subcellular localization, we validated the interaction of LET-756 with RPS-16, FTT-1/PAR-5, FTT-2, KIN-10 and RPL-6 with high score, and with PAL-1, DAF-21, SKR-2, KIN-3 and C18A3.3 with lower strength (see Table 6).

At metaphase, signals were detected at the primary constriction of all metaphase chromosomes in both Aye-Aye and human cells, providing cytological evidence of centromeric localization and validating the use of an antihuman antibody to detect Aye-Aye centromeres (fig. 1 A and B ).

Further, some databases contain experimentally validated localization information whereas others also contain localization predictions.

Other than the protein localization signal databases, a few interesting review articles have also been published (14, 32, 33) which catalog the experimentally validated localization signals, although the proportion of signals reported is much smaller than LocSigDB and typically the reviews are focussed either on a single organelle or on a protein of interest.

The feasibility of the proposed localization scheme is validated by experimental measurements using real resource constrained WSN node prototypes.

The proposed localization scheme is validated by experimental testing of two CFRP plates with cross-ply [0/90]4s and symmetrical stacking [0/45/90/−45/0/90/−45/0]s respectively.

LocSigDB contains 533 experimentally validated localization signals.