This modification regulates protein localization, trafficking, and stability [23] [25].
By studying their intracellular localization, trafficking and assembly, we gained insights about their physiological significance.
We demonstrate that HuR exhibits a remarkably dynamic cytoplasmic localization, trafficking between the CB and polysomes during spermatid differentiation.
Protein palmitoylation plays an important role in regulating localization, trafficking, and degradation of a protein, and its interaction with other proteins.
Intracellular localization, trafficking and protein association of GFP-tagged HFE protein variants were analysed in transiently transfected HepG2 cells by immunoprecipitation and immunofluorescence assays.
Genetically encoded reporter proteins are important and widely used tools for quantification of promoter activity, translational efficiency, and protein localization, trafficking, and stability.
These include organelle organization, chromatin localization, trafficking, cell cycle, protein phosphorylation, and posttranslational protein modification/degradation, among others.
Information concerning localization, trafficking, activation states and interaction partners of proteins in living cells is crucial to understand the complexity of cellular networks.
In these studies, the role of FLNa in augmenting the membrane expression, subcellular localization, trafficking, and signaling of GPCRs is emphasized.
The potential dynamic interplay of E-cad with Numb and Hakai, which awaits further investigation, may be an important mechanism for controlling E-cad localization, trafficking and EMT under physiological and pathological conditions.
It is an enzyme-catalyzed cascade that not only marks protein substrates for 26S proteasome-dependent degradation by covalently conjugating them with multiple ubiquitin monomers via lysine (K 48-linkage, but also alters protein localization, trafficK 48-linkageactivation via K63-linked polyubutuitin chalso [ 56, 57].
