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Usually two nuclear localization sequences (NLS) localized between amino acid residues 320 and 405 were supposed to represent the inter-domain region between MTase and RdRp (Brooks et al., 2002).
In this study, transposon mutagenesis was used to identify localized proteins and short localization sequences in the model bacterial system Caulobacter crescentus.
FAK also contains putative nuclear localization sequences (NLS) within the F2 lobe of its FERM domain and can localize to the nucleus upon receipt of cellular stress, where it binds to p53 (Lim et al., 2008).
Nuclear localization sequences.
Transfection of G6PD knockout cells with G6PD fluorescent mutant proteins demonstrated that the major subcellular localization sequences of G6PD are within the N-terminal portion of the protein.
HSP90 regulates ligand binding, as well as cytoplasmic retention of hGRα by exposing the ligand-binding site and masking the two nuclear localization sequences [4].
Finally, we use the model to predict potential effects of incorporating nuclear localization sequences in these kinds of synthetic vectors, and find that the degree of benefit from these will depend on the values of other key system properties including the vector unpackaging rate constant.
We did not detect any bipartite-like nuclear localization sequences within amino acids 218 to 264.
Fly Nkd also contains nuclear localization sequences and is observed in the nucleus.
Molecular biological analysis identified a bipartite nuclear localization signal, and nucleolar localization sequences in the N-terminal part of STK35L1.
While these nuclear localization sequences are conserved between fly and mosquito Nkd proteins, they are not conserved in vertebrate Nkds.
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