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For detection of the antigen localization, sections were incubated at 4°C for 2 hours with appropriate secondary antibodies.
To assess Tie2 localization, sections were stained with fluorescent antibody recognizing Tie2 (TeBioscienceience, San Diego, CA) or isotype control antibody (IgG1-biotin).
For MMP-9 localization, sections were double-stained with biotinylated rat anti-MMP-9 (R&D Systems, Minneapolis, MN) and with allophycocyanin-conjugated Gr-1, F4/80, or CD169 (eBioscience, San Diego, CA) primary antibodies, followed by streptavidin Alexa Fluor 488 or streptavidin Alexa Fluor 647.
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The 'Source localization' section deals with the source localization issue.
This negative correlation caused us to investigate what would be the effect of suppressing Interfaces from bug localization (Section 4.2.2).
Java has been the focus of previous studies of structured IR-based techniques for bug localization (Section 2.3).
Although they based their results on averaged spikes localization due to the difficulty in localizing the low SNR individual spikes, it is more reliable to perform single spike localization to recover accurate information on the spike onset and propagation (cf. " Why applying fusion to single spike localization?" section).
After a description of acoustic maps for source localization in Section 2, Section 3 presents our approach to the multiple source localization problem.
The sand rat disc was also examined for asporin localization in sections of disc cut en face.
To visualize iNOS localization on sections, an anti-iNOS polyclonal antibody (TCS Biologicals Ltd ,Bucks, UK) was employed at a dilution of 5 μg ml−1.
The "Localization techniques" section describes wireless indoor positioning techniques.
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