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Since the anti-Bmf antibodies currently available are not suitable to distinguish between isoforms by immunofluorescence, we decided to study Bmf localization performing biochemical subcellular fractionation experiments.
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Consistently, these results were confirmed by the confocal microscopy analysis of Numb subcellular localization performed on the same cells.
Co-localization performed on polytene chromosomes showed RNA Pol II and dAGO1 overlapping at some sites.
Unfortunately, inverse algorithms that provide this guarantee were too expensive to use for the large number of source localizations performed for this paper.
Localizations performed with the unmasked genome were not used in the preceding analysis as this had generally only a small negative effect on recall, but had a large negative impact on precision and analysis speed.
We investigated c-FLIP subcellular localization by performing cell fractionation and indirect immunofluorescence.
DS installed the localization algorithms, performed the localizations and participated in the design of the study.
For the few constructs generated in our laboratory, a more detailed analysis of their localization was performed using co-localization experiments with plasma membrane or nucleus markers (not shown).
In our method, since localization is performed by using the linear magnetic map, localization accuracy is not influenced by moving objects.
Thus, OC-based localization method performed better at the sampling rate of 8 kHz (localization failure ≤30%) as compared to 4 kHz (localization failure ≤40%).
But he notes that a relational account of localization could perform such a function, with fields localized relative to each other.
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