Since the anti-Bmf antibodies currently available are not suitable to distinguish between isoforms by immunofluorescence, we decided to study Bmf localization performing biochemical subcellular fractionation experiments.
Co-localization performed on polytene chromosomes showed RNA Pol II and dAGO1 overlapping at some sites.
Unfortunately, inverse algorithms that provide this guarantee were too expensive to use for the large number of source localizations performed for this paper.
Localizations performed with the unmasked genome were not used in the preceding analysis as this had generally only a small negative effect on recall, but had a large negative impact on precision and analysis speed.
DS installed the localization algorithms, performed the localizations and participated in the design of the study.
For the few constructs generated in our laboratory, a more detailed analysis of their localization was performed using co-localization experiments with plasma membrane or nucleus markers (not shown).
In our method, since localization is performed by using the linear magnetic map, localization accuracy is not influenced by moving objects.
Thus, OC-based localization method performed better at the sampling rate of 8 kHz (localization failure ≤30%) as compared to 4 kHz (localization failure ≤40%).
Anatomical localization was performed using a brain atlas [41] and, when possible, statistical localization relied on probabilistic cytoarchitectonic maps [42].
