However, the low number of protofilaments observed in this study is difficult to reconcile with the expected number (20 80 protofilaments) calculated from the concentration and midcell localization percentage of FtsZ in C. crescentus [23].
For example, in a different strain (AG1) we found that the midcell localization percentage of FtsZ is near 100% because we observed the formation of new Z-rings immediately following cell division.
Frequencies for localization are shown in Table 2. Table 2 Tumour localization (number of patients counted (n) = 1484 Localisation Percentage Floors of the mouth (144) 42 Tongue (141) 25 Alveolar gingiva (143) 17 Other nonspecific sites (145) 145
(H ) Calculated percentage of DNA-bound TERT monomer complexes according to fraction TERT subunit co-localization (percentages indicated), assuming the TERT-labeling efficiency measured value (82%, blue line; bar graph at right ), lower bound (51%, green line; Low L numbers at right ), or upper bound (100%, purple line; High L numbers at right ).
The PPP1CC signal was particularly decreased in both the MTOC and in microtubules, suggesting that TCTEX1D4 is at least partially responsible for PPP1C MTOC/microtubular localization/transport. Results were further confirmed by quantitative correlation analysis of PPP1CC/TCTEX1D4 co-localization percentages in the cytoplasm of TCTEX1D4 transfected cells (Fig. 7D).
Table 1 shows the digital quantification of the confocal immunofluorescence analysis, with the mean of intensity and co-localization percentage.
More selective co-localization criteria, i.e. considering only the ring-like pattern of EGFP-MC-LT staining, led to a reduction in the co-localization percentage (45 50%), however, no differences were found between wild-type and Oa1-deficient cells.
The details of KIT staining intensity, pattern, localization and percentages of immunopositive cells are given in Table 2. Moderate KIT staining was seen in 2/7 meningioma cases, while, strong expression was detected in 2/7 cases.
Two independent investigators assessed the subcellular localization and percentage of stained cells for each sample.
To ascertain if the wsc1 ∆ mat formation defect was attributable to a defect in Flo11p expression or localization, the percentage of cells expressing Flo11p on the cell wall were measured by immunofluorescence.
In addition to membrane localization, a small percentage of the EGR receptors were localized to the cytoplasm basally when co-expressed with HER2 in HEK293 cells.
