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Because of this localization, changes of the local dimensions are largest in the neck, where the local strain exceeds the macroscopic strains.
Taken together, the data reported above demonstrate that Numb localizes in the nucleus of early thymocyte precursors and its nuclear localization changes over time, where the appearance of pre-TCR signaling at DN3 stage promotes nuclear exclusion.
In reality, interactions can depend on protein modifications, localization changes, co-expression of other proteins, and environmental cues.
We used C. elegans strains transgenic for the SMF GFP reporters to follow DMT1-like protein expression and localization changes upon Mn exposure.
Taken together, these results indicate that Trx-CFP and TrxR1-YFP are reliable reporters for dynamical localization changes of the wild-type Trx and TrxR1.
Second, we showed that SIRT1 localization changes after treatment with H2O2, an effect blocked by treatment of cells with a JNK1 inhibitor.
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Others, using similar GFP-BAX constructs, had demonstrated that BAX localization changed from a diffuse labeling pattern to more punctate foci, co-localizing with mitochondrial markers, as active protein translocates and aggregates to the MOM [ 22, 23].
A significant requirement for a bone implant is to replicate the functional gradient across the bone to mimic the localization change in stiffness.
Interestingly, Sln1p-YFP and Nik1p-YFP fusion protein localization changed in response to hyperosmotic stress by rapidly clustering into punctuated structures that could be associated to osmotic stress signaling.
To further explore the role of hemocytes in antiviral responses, we next determined whether their localization changed during CrPV infection.
Taken together, the expression of ZAS3 was increased and its subcellular localization changed from nuclear to cytoplasmic upon osteoclastogenesis of RAW 264.7 cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com