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A subcellular localization assay indicated that OsNAP is a nuclear-localized protein.
a Subcellular localization assay.
DAF-16:: GFP localization assay were performed as described previously [40].
Shaanhe 6. Subcellular localization assay showed that TaDlea3 protein accumulated in the cytoplasm.
Subcellular localization assay showed that CfIAP1 was mainly distributed in cytoplasm and CfIAP2 was in cytoplasm and nucleus.
We have used the binding pair to develop a channelrhodopsin membrane localization assay that is amenable to high-throughput screening for opsin discovery and engineering.
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Localization assays revealed that CiNAC3 and CiNAC4 localized in the nuclei, consistent with their roles as transcription factors.
Plasmids pRTVnGFP, pRTVnYFP-OsRac1, and pRTVnRFP-WRKY45 were used for subcellular localization assays.
For nuclear localization assays, aliquots of 106 cells in 500 μL of growth medium were exposed to 111In-BnDTPA-F3 111In-BnDTPA-F3 111In-BnDTPA-F3.
Using the data obtained from nuclear localization assays, time-activity curves were generated for each of the compartments of the cell: membrane, cytoplasm, and nucleus.
The results indicated that these binary vectors worked properly in rice, and that they can be used in rice transgenic experiments, including gene overexpression assays, tissue or cell localization assays, promoter activity assays, and complementation assays.
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