Co-localisation was quantified using a mask generated from the streptavidin channel as explained in Figure 2 figure supplement 3. Importantly, as the different markers label abundant structures it was possible that low levels of co-localisation would be detected due to chance overlap rather than specific labelling of the same structures.
The key high-density condition, which is most similar to that of Evans et al. (2013, 2016), gives no evidence for there being more 'detection without localisation' trials than would be predicted to be lucky guesses.
A simple explanation for the localisation of DivIVA would be that this protein is targeted by another protein; possibly a membrane protein.
VDR presented a nuclear localisation, as it would be expected for a nuclear receptor, while CYP27B1 and CYP24A1 enzymes displayed cytoplasmic staining with a granular pattern, which is consistent with their mitochondrial localisation.
Detailed investigation of the cellular localisation of mTHPC within neurons would be a useful future direction as differences in drug distribution between neurons and other cells might account for the differences in sensitivity reported here.
A change in localisation of Hsp90 in tumours would be predicted to change the pattern of bound Hsp90 clients and may contribute to the specificity of 17-AAG for tumour cells.
Given that DivIVA binds to lipids, a striking possibility would be that the localisation of this protein depends on the localisation of certain lipid molecules.
We have no evidence that for this high-density condition the number of observed 'detection without localisation' trials is more than what would be predicted by 'lucky' guesses.
Therefore, standardizing CT reports to include nodule characteristics (size, localisation, spiculation, calcification, and mediastinal node) would be useful.
Further studies on the subcellular localisation of the Δ11b splice variant would be required to address this.
Given the localisation close to a fascia ossifying myositis would be benign second differential diagnoses.
