Exact(2)
Moreover, our localisation study provides convincing evidence that the antigen recognised by monoclonal antibody YL1/2 at trypanosome mature basal bodies is FOP family protein TbRP2, not tyrosinated α-tubulin as widely stated in the literature.
To guarantee comparable cure rates with MIP, the latest propositional statements recommend the use of iPTHa during targeted parathyroidectomy for patients with a single (or two non concordant) preoperative localisation study [ 27].
Similar(58)
Here, we show that both mRFP variants can also be applied for localisation studies in other organisms.
Localisation studies reveal that two of the enzymes are found predominantly in a parasite-specific organelle, the glycosome (TbSODB1 and TbSODB2), while the other two are targeted to the mitochondrion (TbSODA and TbSODC).
For simple localisation studies, the cells were incubated with 0.6 or 1 mM of GdBN functionalised with cyanine 5.5 used as a fluorescent marker (GdBN-Cy5.5) during different incubation times (1 hour, 6 hours and 12 hours).
In order to confirm this, we performed in vivo localisation studies using 35S promoter-driven GFP fusion constructs of AHK5.
Given the observed interactions between p55 and gelsolin we undertook localisation studies of gelsolin in the stereocilia bundle and at the apical hair cell surface.
Interestingly, our localisation studies suggested that there is a correlation between MG/BC phenotype upon hit depletion, and their localisation to the membrane of the early secretory pathway (ER, tER sites and Golgi complex).
The sensitivity of both mutant lines to H2O2 was restored by the expression of the wild type AHK5 cDNA under the control of the 35S promoter (Fig. 4C) showing that the GFP-AHK5 fusion protein used for the localisation studies is functional in planta.
SV carried out the localisation studies.
Localisation studies of proteins are important indicators of function.
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