Sentence examples for localisation studies from inspiring English sources
Here, we show that both mRFP variants can also be applied for localisation studies in other organisms.
Localisation studies reveal that two of the enzymes are found predominantly in a parasite-specific organelle, the glycosome (TbSODB1 and TbSODB2), while the other two are targeted to the mitochondrion (TbSODA and TbSODC).
For simple localisation studies, the cells were incubated with 0.6 or 1 mM of GdBN functionalised with cyanine 5.5 used as a fluorescent marker (GdBN-Cy5.5) during different incubation times (1 hour, 6 hours and 12 hours).
In order to confirm this, we performed in vivo localisation studies using 35S promoter-driven GFP fusion constructs of AHK5.
Given the observed interactions between p55 and gelsolin we undertook localisation studies of gelsolin in the stereocilia bundle and at the apical hair cell surface.
Interestingly, our localisation studies suggested that there is a correlation between MG/BC phenotype upon hit depletion, and their localisation to the membrane of the early secretory pathway (ER, tER sites and Golgi complex).
For co-localisation studies, U87 cells were incubated with Lysotracker-green (Invitrogen) (75 nM) or Mitotracker-green (Invitrogen) (200 nM) dissolved in HBSS and mixed with DMEM for 45 minutes.
The nanoparticles with the lower Nile Red content were used in all experiments except for co-localisation studies between nanoparticles and early endosomes, in which particles with the higher Nile Red content were used to achieve similar fluorescence intensity as the labelled early endosomes.
For co-localisation studies cells plated onto glass coverslips were transfected with 1 µg of each construct.
In co-localisation studies with mitochondrial markers we have ascertained that CaBP7 and CaBP8 do not traffic to these organelles (unpublished data).
For co-localisation studies twelve-bit images were collected, using the same palette between images, at optimal settings, with 63 x oil immersion lens (NA 1.4).
