The need to utilise In to visualise antibody tumour localisation raises the issue of how many antibodies have been prematurely aborted from further development based on initial results from biodistribution studies undertaken using radio-iodine-labelling and suggests that negative results obtained thusly should always be confirmed with a radiometal-based nuclide.
This pattern of co-localisation raised the possibility that FLRT1 could be a potential substrate for ligand-mediated FGFR1 phosphorylation which could, in principle, regulate FLRT function.
In order to study its localisation, we raised antibodies and generated constructs to express GFP-tagged versions of full length Bug22.
The effect of spreading of HEKs on [T] shapes was studied using phalloidin- and immuno-staining for F-actin networks and focal adhesion localisation (using primary antibodies raised against vinculin, Fig. 8).
In order to investigate subcellular localisation in T. brucei, we raised a polyclonal antibody against full length recombinant protein.
As both proteins are somewhat similar in sequence to syntaxin 16 (see Additional file 2), which is a Golgi syntaxin, and as the Golgi apparatus is known to be located at the anterior part of the cell in kinetoplastids, we performed co-localisation studies with an antibody raised against T. brucei Rab1, a characterised kinetoplastid Golgi apparatus marker [ 44].
The cell-type dependent localisation of endogenous dSmyd4 raises interesting questions about how the localisation of dSmyd4 is regulated.
To investigate whether nutrient induced change in CTPsyn localisation was conserved in animal tissues in vivo, we raised larvae on food for 24 hours, transferred them onto minimal food for 52 hours, and then dissected.
Supershift reactions were carried out by pre-incubation of nuclear extracts with an excess of p50 antibody (goat polyclonal IgG raised against a peptide mapping inside the nuclear localisation sequence) and p65 antibody (goat polyclonal IgG mapping within the amino terminal domain).
43 44 Furthermore, several different morphological forms were noted after eradication of H. pylori as (a) flat, (b) raised, (c) haemorrhagic and (d) appearing as bleeding spots with localisation in the antrum in the absence of drugs, 45 possibly owing to hyperacidity after eradication therapy.
For example, mRNA transport and localisation is highly reproducible, raising doubts about whether the underlying cytoskeletal organisation can be mostly disordered.
