Growing the CreA::GFP strain overnight in glucose (100% nuclear localisation) and then exposing it to carbon starvation for 5 h (~0% nuclear localisation) enabled the study of the positive signals for CreA repression.
Localisation of the interaction is best studied with BiFC, whereas multiprotein dynamics are possible for competitive (multicolour BiFC) and cooperative (BiFC/BRET) interactions.
A larger patient population, with more variation of the number and localisation of the DIL, has to be studied to extend these preliminary tumour control and estimates.
Therefore, to obtain information about the spatio-temporal control of these regulatory proteins, it is essential to study the localisation of the proteins themselves.
Next, we wanted to study the localisation of the different XRCC1-complexes in vivo using live, freely cycling cells expressing XRCC1-EYFP.
In the present study, the localisation of the Runt-related transcription factor-2 (Runx2), its transcriptional activity, as well as its regulation of expression was analysed in human pancreatic ductal adenocarcinoma (PDAC).
Significant correlation was also not observed between the PRL mRNA expression and immunohistochemical localisation of the PRL protein in the previous study by Bhatavdekar et al. [ 13], indicating that the presence of PRL mRNA does not necessarily point to the presence of the PRL protein.
To investigate the role of Cep126, we first studied the localisation of the endogenous protein using confocal microscopy.
To study the cellular localisation of the peptide aptamers immunocytochemistry (ICC) was used to probe for pepBim, pepPuma and empty SQT in Hela cells expressing these peptide aptamers.
To address a possible role for the WxxxE motif in interaction of BtpA with microtubules, we performed ectopic expression analysis to study the subcellular localisation of the wild type protein and a panel of variants.
