Co-localisation of samples in the blot is indicative of a strong association.
To determine the subcellular localisation of NOS, a sample containing only cytosolic proteins of the crude homogenate was analysed.
Due to the often focal localisation of the disease, sampling error may occur.
We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4 9) and in 39 specimens of benign prostatic hyperplasia (BPH).
Immunohistochemistry was also used to examine localisation of EphB4 in tissue samples from 15 patients with prostate carcinomas.
Amplification of the STAT5A/B locus has been described in prostate cancer, and is associated with increased expression and nuclear localisation of STAT5 in tumour samples.
The sensitivity of ASVS is comparable to portal venous sampling for localisation of insulinoma and better than portal venous sampling for localisation of gastrinomas [ 28, 29].
However, none of these studies has shown any statistical correlation between these molecules and subcellular localisation of β-catenin in tissue samples (Karbova et al, 2002; Takada et al, 2004).
In addition, it allows for the localisation of sequences to pathology in tissue samples.
Therefore, to determine whether myosin Vb protein is involved in the localisation of Rab11a, we compared small intestine samples from a microvillus atrophy patient and a healthy individual (Fig. 6C), as microvillus atrophy patients are known to have mutations in the myosin Vb gene (Müller et al., 2008).
Further study by Kouri et al. (2013) compared RA and OA synovial tissue, using immunohistochemistry to show cytoplasmic localisation of BMAL1 in immune cells and fibroblasts in RA samples, while staining was nuclear in OA samples.
